EVALUATION OF BIOLOGICAL ACTIVITIES FROM THAI HERBAL LOZENGES

Authors

  • Yuttana Sudjaroen
  • Kanittada Thongkao
  • Narin Kakatum

DOI:

https://doi.org/10.47750/pnr.2022.13.03.018

Keywords:

antitussive, biological activity, expectorant, herbal lozenge, Thai herbal medicine.

Abstract

UM-MA-RUEK-KA-VA-TEE (UM) is multi-herbal formula as antitussive and expectorant. New UM formula was prepared in lozenge for simply use. The research was proposed to evaluate bioactive constituents and biological activities of lozenge herbal mixture, including liquorice root, Indian gooseberry, coriander seed, gall of chebulic myrobalans, cumin, belleric myrobalan and karanda fruit. Phenolic, flavonoid and saponin contents were determined by spectrophotometric methods. Antioxidant activity was evaluated by DPPH and NO radical scavenging and reduction of lipid peroxidation. Anti-inflammation was evaluated by inhibition of albumin degradation and NO production of LPS-induced macrophages. Cytotoxicity was evaluated against human dermal fibroblasts. Herbal mixture (10%) and other ingredients was performed to lozenge. Reduction of oral microbials was determined in healthy volunteers (N = 30) before and after use lozenges by a modified dip-slide test. Total phenolic, total flavonoid and saponin contents were 188.34±2.96 mg of GAE/g, 19.24±1.59 mg of QE/g and 534.80±34.18 mg of SE/g, respectively. Herbal mixture was strongly inhibited DPPH and NO radicals, and potently reduced NO production from macrophages and without cytotoxicity. Oral microbials, including Mutans streptococci and Candida sp. were significantly decreased after lozenge use. New UM formula was contained saponins as most bioactive compound, and antioxidant, anti-inflammation and anti-microbial activities was preferable.

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Published

2022-09-20

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Section

Articles

How to Cite

EVALUATION OF BIOLOGICAL ACTIVITIES FROM THAI HERBAL LOZENGES. (2022). Journal of Pharmaceutical Negative Results, 13(3), 116-120. https://doi.org/10.47750/pnr.2022.13.03.018