Formulation Development and Evaluation of Herbal Nanoparticles containing Ointment of Leaves extract of Rhynchosia rothii

Abstract

In our previous work we have reported wound healing activity of leaves extract of Rhynchosia rothii in which we got excellent
activity. Therefore in present study, the development of Rhynchosia rothii loaded Lycoat RS 720-BSA conjugated polymeric
nanoparticles and subsequent ointment formulation has been aimed. Maillard Reaction was used to develop the Lycoat RS 720-BSA
conjugate. The solvent evaporation approach was used to produce nanoparticles with Rhynchosia rothii loaded on them. The
nanoparticles had a 257nm particle size and exhibited a spherical shape. The zeta potential in the formulation was -22.4 mV.
Entrapment efficiency was 88.32% in the enhanced batch. The extracted content of the optimized batch was found to be 78.10%. A
continuous release pattern was found by the in-vitro diffusion investigation, with 94.24% diffusion after 24 hours. The Rhynchosia
rothii extract was created as an absorbent ointment formulation with a pH of 6.80–6.92 and a spread ability of 80.00–110.16 g.cm/s.
It also has a distinctive aroma. Ointment containing herbal nanoparticles from the F4 batch has produced positive results. A new
method of promoting nanoparticles in herbal medication delivery systems is by employing them in nanoparticles and an ointment.
From present investigations we concluded that prepared ointment can be used clinically for the treatment of wounds if optimized
further using more in vitro and in vivo models along with toxicity predictions.