Implementation Of Box Behnken Design To Fabricate The Formulation, Optimization And Dissolution Of Blueberry Nanosuspension

Abstract

The present study aims to enhance the bioavailability of Blueberry by formulating it as nanosuspension. Blueberry mainly consists of
anthocyanins which have strong antihyperlipidemic activity but suffers from low bioavailability which causes hindrances in its
therapeutic activity to treat hyperlipidemia. Hence to improve its bioavailability, nanosuspension of blueberry was prepared. BoxBehnken design was applied to predict the effect of independent variables namely concentration of chitosan and concentration of
tripolyphosphate (TPP) and stirring speed on two responses namely particle size and entrapment efficiency. The design suggested that
a formulation having a particle size of 205.05nm and entrapment efficiency of 76.60 % will be an optimized formulation if it has
chitosan, TPP,and stirring speed of 0.5%, 0.05%,and 1050 rpm respectively. The formulation suggested by design was prepared and it
was found that particle size and entrapment efficiency of nanosuspension 198.5 nm and 75.14±0.56 % respectively. In addition, it was
found to have polydispersity index, refractive index, zeta potential of 0.351, 1.33, 21.08mv respectively.SEM analysis showed the
droplet size under 200nm.When compared towards blueberry nanosuspension with drug extract release it shows the controlled release
of drug up to 8 hrs which enhances the bioavailability of drug and drug extract suspension showed the maximum release in 2hrs which
shows the immediate release of the drug. As a result, our research shows that nanotechnology might be utilized as an effective approach
increasing the bioavailability of blueberry nanosuspension and this article also focuses on the antihyperlipidemic activity of
anthocyanin.