Biosynthesis Nanoparticles And Molecular Study To Detect Bacterial Isolated From Patients With Diabetic Foot Ulcers In Babylon Province

Abstract

Objective:- Diabetic foot ulcers, the aim of the study for determine some types of microorganisms from diabetic foot ulcers that
causes inflammation and long-term complications and activity study of ZnO NPs biosynthesis by Aspergillus niger against isolated
bacteria, collected from infected patients at general Al -Hashimiya hospital in Babylon Governorate.
Methods:-collected 100 swabs, cultured it, bacteria was diagnostic by morphological and microscopic, standard biochemical tests and
Vitec,the isolates identified by molecular diagnosis by extraction of DNA,using specific universal primer 16SrRNA (1239bp) and
polymerase chain reaction (PCR), study biosynthesis,characterization of zinc Oxide Nanoparticles to inhibited bacterial growth.
Results:-The results were (26 aerobic bacterial isolates positive) present such as Gram positive Staph aureus , as regards the aerobic
Gram negative bacilli, Klepsiella pneumoniae, Proteus mirabilis, Escherichia coli, The zinc oxide nanoparticles created by
Aspergillus niger were spherical in shape with size 13.5nm that used as antibacterial.
Discussion:-Through determination of MIC were concentrations of ZnO NPs (31.25, 62.5μg /ml, 125μg/mL, 250μg/ml, 500μg /ml),
(31.25, 62.5μg /ml) not prevent growth and not inhibition while 125μg/mL determine as MIC, two best concentrations are 250μg/ml,
500μg /ml both give inhibition zones.
conclusion:- Gram negative bacteria was predominantly for diabetic foot ulcers, Aspergillus niger was shown to be a stabilizer
capable of producing spherical ZnO nanoparticles, due to is very suitable candidate to carry out antimicrobial operation, as a result to
its small size.