Molecular detection of Toxoplasma in women with abortions

Abstract

The current study aims to molecular detection of Toxoplasma in women with abortions. Seventy-five aborted women visited the private clinics between 2021 and 2022. Each female filled out a questionnaire that asked about her age, address, blood type, how many abortions she had, how many cats she had at home, and if she was a carrier. Venous blood samples (5ml) were carefully drawn into EDTA tubes and centrifuged at 300 rpm for 5 minutes to extract the clear serum. The sera were then analyzed to determine the amount of antibodies against T. gondii infection. The Latex Agglutination Test, Cassette IgG\IgM combo rapid test were done for detection of toxoplasmosis. PCR was done for detection of B1 gene that is representative for toxoplasma diagnosis. The current results showed that the positive results of latex agglutination test were significantly higher in results than Cassette IgG\IgM combo rapid. The findings revealed 49 (63.3%) for IgG and 11 (14.7%) for IgM. Out of the 75 pregnant and aborted women who were 34 (45.3%) instances in the study were PCR positive.
In conclusion, PCR was the gold test for diagnosis of toxoplasmosis.