Effect of Different Doses of Sorbitol on Hematological Parameters and Blood Glucose of Rats

Abstract

The current study was conducted in the animal house of the college of veterinary medicine, Al-Qasim green university during December (2021) to February (2022) to investigate the effect of sorbitol as one of the most worldwide used chemical sweeteners on hematological parameters and on blood glucose. For this purpose, a total 40 male Wistar rats of 60 days age old and 300±10 grams weight were used, acclimated divided randomly to 4 groups [control (GC) given distilled water and 3 experimental tested (T1, T2 and T3) which given respectively 50, 100 and 200 mg/kg B.W of sorbitol for 60 days], and finally subjected for direct collection of blood samples. Using the automatic blood analyzer, the findings of hematology were revealed a significant increase in values of red blood corpuscles (RBCs), packed cell volume (PCV), hemoglobin (Hb), white blood cells (WBCs), lymphocytes, neutrophils, monocytes, eosinophils and basophils of all treated groups when compared to value of CG. Among values of treated groups, significant higher value was reported in T3 when compared to those of T1 and T2. The results of blood glucose were noted a significant elevation in values of treated groups when compared to value of CG. Moreover, there was an ascended elevation in values of blood glucose throughout the treated groups since significant higher level was reported in T3 and then in T2 and T1 values. In conclusion, the findings of this research sound a cautionary note about the impact of sorbitol on RBCs and blood glucose. However, we recommended to extending the scope of investigations to include all relevant aspects.