In Vivo, Molecular Evaluation for Improvement of Sperms Mitochondrial Functions by Fenugreek, Extract and Nanoparticles

Authors

  • Mohammed T. Jaafar
  • Hayder A. N. Al-Zamely
  • Adnan Mansour Jasim

DOI:

https://doi.org/10.47750/pnr.2022.13.S01.210

Keywords:

Testis, SDHA gene, Chitosan, NP, PCR, Iraq

Abstract

This study was carried out to investigate the possibility of using the fenugreek extract and fenugreek NPs to improve sperms mitochondrial functions in rats through estimation the level of testicular protein using the Real-Time polymerase chain reaction (RT-PCR). Totally, 60 Wister rats were selected, prepared and equally divided into three groups; negative control that remains without any treatment and given only distilled water daily; T1 that orally treated with a daily dose (100) mg/kg of extract fenugreek, and T2 that orally treated with a daily dose (100) mg/kg of fenugreek NPs. After an experimental period continued for 60 days, all study animals were euthanized with chloroform and collection of testis samples that subjected for RT-PCR examination through targeting SDHA, CS, and GAPDH genes. The findings revealed that the Ct of the calculated genes were increased in SDHA-fenugreek and CS-fenugreek when compared to GAPDH-fenugreek; as well as in values of SDHA-NPs and CS-NPs in comparison with GAPDH-NPs. Additionally, values of SDHA-control and CS-control were significantly higher than those observed in GAPDH-control. Regarding the fold change of genes, significant elevation (P0.05) was reported in values of fenugreek NP in both SDHA and CS genes against the control as well as the fenugreek extract. In conclusion, Analysis of mitochondrial activity can give important additional information on the quality of a given spermatozoa sample, and highlights the importance of genetic analysis in confirming the expression of SDHA and CS genes. Additionally, the administration of both fenugreek extract and fenugreek NPs was revealed in a significant increasing in protein expression with a more significant effectiveness of fenugreek NPs rather than fenugreek extract. However, we recommended that the augmentation of fenugreek NPs and the proper regulation of steroidogenesis and mitochondrial biogenesis related genes is notably need to be furthermore studied. The toxicity of chitosan NPs needs to be evaluated, in particular in embryo development aspects, because extensive research into their biomedical applications was based largely on the biodegradable and biocompatible profile of chitosan.

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Published

2022-10-03

Issue

Section

Articles

How to Cite

In Vivo, Molecular Evaluation for Improvement of Sperms Mitochondrial Functions by Fenugreek, Extract and Nanoparticles. (2022). Journal of Pharmaceutical Negative Results, 1758-1764. https://doi.org/10.47750/pnr.2022.13.S01.210