Size Control of Ag Nanoparticles Synthesized by PLA Method in Different Liquid Environments and their Potent against Virulent Candida Albicans

Abstract

Human candidiasis is mostly caused by Candida albicans. It is now one of the most prevalent, recurrent and drug resistant forms of fungal infection, especially vaginal candidiasis. This research examined the antifungal activity of silver nanoparticles AgNPs on the viability of C. albicans. Pulsed laser ablation was used to produce silver nanoparticles in liquids in diverse liquid environments, with a Nd-YAG laser pulsed Q-switched wavelength (1064nm), number of pulses about (1000) pulses, and the repetition rate of (1)Hz. UV-VIS and TEM techniques were used to determined the optical and morphological properties of the produced colloidal nano-solutions. UV-VIS exhibited a characteristic peak at the site of the surface plasmon peak (SPR) at (400 nm) in double deionized distilled water (DDDW), and this value increased to (407, 408 and 415 nm) when used the solutions (SDS, CTAB and NaBH4), respectively. The morphological properties TEM of Ag NPs were distinguished, including particle size and shape where indicating that the shapes of spherical nanoparticles are heterogenic with an average size ranging (41.198- 50.631) nm. In addition, the current investigation revealed that AgNPs possess excellent and considerable antifungal activity, and that the rate of inhibition of C. albicans gradually increases as concentrations rise. The genotoxicity indicated a decrease in values of (BI), (MI) with increases concentrations. This drop is attributable to the interaction of (NPs) with cellular structures. While the value of (TCA) only slightly rose at concentration (75µg/mL), it was demonstrated that silver nanoparticles could change cell structure.