THE IMPACT OF FOLLICLE STIMULATING HORMONE RECEPTOR GENE POLYMORPHISM ON PROGRESSION OF PRIMARY AMENORRHEA IN A GROUP OF IRAQI FEMALES: A CASE CONTROL STUDY

Abstract

This research designed to investigate the impact of single nucleotide polymorphism (rs6166) of the Follicle Stimulating Hormone Receptor (FSHR) gene in a group of females diagnosed with primary amenorrhea. Blood samples were collected from healthy control group consisted of 40 females between the ages of 12-35 who appeared to be in good health, while 62 patients who visited the educational laboratories at the City of Medicine in Baghdad from October 2018 to February 2019. In the meantime, molecular analyses were performed on all blood samples. SNP (rs6166) determinations were performed using direct sequencing. Calculations of allele frequency for exon10 rs6166 single nucleotide polymorphism showed that G and A alleles was not associated with the disease with P value (0.306) for both alleles and also showed no significant association for the AA , AG and GG genotypes with the disease with P (0.650), (0.675) and(0.261) respectively. Comparing between genotype variations and hormones level in serum in control group showed a significant association of AG genotype with FSH level with P value(0.031), while in patient group the comparison showed significant association of AA and AG genotypes with LH level. The comparison of age categories with genotype variations for both control and patient groups showed no significances with P >0.05. All these results confirmed that rs6166 polymorphism could not considered as a molecular marker for primary amenorrhea in Iraqi females.