The Effect Of Endurance Exercise Training, Along With The Consumption Of Black Mulberry Leaf Aqueous Extract, On Heart Contractility In Overweight Rats

Abstract

Aim: Endurance exercise is used as one of the clinical interventions in the prevention and treatment of cardiovascular diseases. Black mulberry leaves (Morus Nigra) containing phenolic compounds that have pharmacological properties are used to lower blood pressure and lower cholesterol. The aim of the present study was to evaluate the effect of black mulberry leaf aqueous extract alone and its combination with physical activity on heart contractility in overweight rats.

Method: 35 male Wistar rats were divided into 5 groups with free access to water and food. The control group was fed with normal food and 4 groups were fed with 60% high fat food for 12 weeks to cause hyperlipidemia. Then, endurance training and gavage of black mulberry leaf aqueous extract were performed for 6 weeks, 5 days a week. At the end, using the Power lap electro physiograph system, heart contractility indices, including heart rate (HR), arterial systolic pressure (SP), diastolic pressure (DP), left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) The maximum rate of left ventricular pressure increase (+dp/dt max) and the maximum rate of left ventricular pressure decrease (dp/dt max -) were recorded.

Findings: The maximum rate of left ventricular pressure increase (dp/dt max +) and the maximum rate of left ventricular pressure decrease (dp/dt max-) in the exercise training group showed a significant increase compared to the normal diet control group and the high-fat diet control group (P< 0.05) and (P<0.001). These indicators showed a significant decrease in the extract consumption group compared to the control group and the exercise group (P<0.001) and (P<0.01). In the extract and exercise group, both indicators showed an increase compared to the extract group, but did not reach the level of the control and normal groups. Left ventricular systolic pressure (LVSP) and left ventricular end-diastolic pressure (LVEDP) showed a significant decrease in the extract group compared to the exercise group (P<0.01).

Conclusion: Consumption of black mulberry leaf extract caused heart muscle relaxation and decreased (dp/dt max +) and (dp/dt max -) compared to the control group. Adaptation to sports training and improving cardiac muscle contraction function partially compensated for this decrease and improved the decrease in cardiac function caused by the effect of black mulberry leaf extract.