In vitro micropropagation of Alternanthera Ficoidea L. by callus formation on MS medium


  • Jesús María Zambrano Salas
  • Keny Davi Alvarado Quiroz
  • Xiomara Vanessa Bustinza Fernandez
  • Rosmery Cárdenas Hurtado



in vitro propagation, disinfection, phytohormones and acclimatization


In this research work, the in vitro propagation of Alternanthera ficoidea, an ornamental plant belonging to the Amaranthaceae family
native to South America, was carried out. This plant is considered important due to the presence of important pharmaceutical products
such as tannins, saponins, phytols, carotenoids, xanthophylls and polyphenols, which is why it can be included as a medicinal herb as
well as being used colloquially as a tranquilizer and as a treatment for gastritis. This method guarantees to obtain a large number of
homogeneous explants from the mother plant tissue, taking advantage of the division and regeneration capacity that plant cells possess
when they are in adequate conditions for their growth. To achieve this in vitro establishment, three disinfection methods were evaluated,
obtaining a greater number of viable explants with the use of 0.1% mercuric chloride for 5 min and greater shoot growth with the
combination of phytohormones (1.5 + 1.5 mg.L-1 of AG3 and BAP respectively) reaching an average shoot length of 3.7 cm. These
seedlings were acclimatized to nursery conditions.







How to Cite

In vitro micropropagation of Alternanthera Ficoidea L. by callus formation on MS medium. (2022). Journal of Pharmaceutical Negative Results, 13(3), 785-788.