Evaluation Of Photoactivated Curcumin Loaded Self Emulsifying Drug Delivery System In In Vitro Microbial Caries Model Against Enterococcus Faecalis

Abstract

Self-emulsifying drug delivery system (SEDDS) has the potential to provide a platform/cargo for such hydrophobic molecules (curcumin) to utilize the full spectrum of such molecules. In this study we have evaluated the antifungal potential of Curcumin loaded SEDDS against Enterococcus faecalis in dental caries model. Two curcumin loaded SEDDS formulations were prepared containing different excipients. Both formulations were physiochemically assessed through zeta sizer. After characterization the antifungal activity of the formulations were determined by agar well diffusion. Afterwards minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), in vitro biofilm and antibiofilm assay were performed both in visible light and dark conditions. Both the curcumin loaded SEDDS formulations were physiochemically accessed and showed good PDI, zeta potential and zeta size values respectively. Both the formulations showed excellent antifungal activity by making zones of inhibition and MICs. MIC and MBC values of both the formulations were same which showed that curcumin loaded SEDDS is bactericidal at lowest concentration. Photoactivated curcumin loaded self-emulsifying drug delivery system is a promising alternative of antibacterial to treat dental caries causing and biofilm producing isolates of E. faecalis with least amount of cytotoxicity.