A Saliva's Physicochemical Characteristics And Total Salivary Antioxidants Ex Vivo Research Evaluating The Effect Of Probiotics

Abstract

BACKGROUND: It has long been proven that oxidative stress plays a role in the pathogenesis of several illnesses. In addition to acting as free radical scavengers and preventing cell damage, antioxidants also have an impact on the metabolism of a number of pathogenic bacteria. Alternative therapeutic treatment alternatives are becoming more prevalent as antibiotic resistance rates rise. Probiotics, according to the WHO, are the second-most important immune defense mechanism after antibiotic resistance. Probiotics' specific mode of action is still up for debate, despite several research emphasizing their beneficial effects on illnesses. The goal of the current research was to reveal the probiotics' antioxidant properties in saliva as well as how they affected the pH and buffering power of the saliva.

METHODS: The study was conducted in PAC Hospital Kamra. Before and after consuming probiotics for two weeks, unstimulated saliva from 10 people was tested for total antioxidant content, pH, and buffering capacity using a spectrophotometer, pH paper, and buffer strips, respectively. For this research, healthy adults between the ages of 20 and 35 were included; those who often smoked or drank alcohol as well as those with any systemic diseases were excluded. After 14 days of probiotic use, the whole procedure for determining the pH, buffering capacity, and total antioxidant content in saliva was carried out, and comparisons between the three parameters were made.

RESULTS: To check for changes, the total antioxidant content, pH, and buffering capacity were tested before and after for the whole sample. While pH and buffering capacity showed negligible findings with p-values of 1.00 and 0.08, respectively, total antioxidant level demonstrated significant outcomes with a p-value of 0.003. The overall findings demonstrated that probiotic consumption increased the amount of total salivary antioxidants without significantly affecting pH or buffering capacity. The "paired t-test" was used to statistically assess the results.

CONCLUSIONS: Probiotics are useful for boosting antioxidant levels, which reduces cellular damage and makes them less likely to cause illness. Additionally, an increase in antioxidant levels may be readily detected in saliva, making it a useful diagnostic tool.