Phytochemical Investigation And Determination Of Total Phenols, Flavonoid, Saponins And Alkaloids Concentration In Root Extract Of Tabernaemontana Divaricata R.Br. Ex Roem. & Schult

Abstract

Bio-efficacy of plants and their derivatives have been reemphasized in recent times. In the last few years, there has been an exponential growth in the field of herbal medicine and gaining popularity both in developing and developed countries because of their natural origin and less side effects. Medicinal plants continue to be an important therapeutic aid for alleviating ailments of humankind. The aim of the present study was to evaluate qualitative and quantitative phytochemical analysis of roots of Tabernaemontana divaricata (T. divaricata) collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol, flavonoids, saponins and alkaloids were determined by the well-known test protocol available in the literature. Phytochemical analysis revealed the presence of alkaloids, glycosides and flavonoids. Quantitative analysis of phenolic and flavonoids was carried out by folins ciocalteau reagent method and aluminium chloride method respectively. It is expected that the important phytochemical properties recognized by our study in the indigenous medicinal plant will be very useful in the curing of various diseases when taken along with our food. Further investigation regarding isolation and purification of a number of phytoconstituents from different parts of T. divaricata may yield optimal combinations of therapeutic alternates. The findings of the present study will be helpful to phytochemists, pharmacologists and pharmaceutical industries.