Formulation, Development And Evaluation Of Polyherbal Formulation For Antispasmodic Activity

Abstract

Irritable bowel syndrome (IBS) is a symptom of functional gastrointestinal disorders. It is commonly experienced as abdominal cramp, diarrhea, and constipation. The diagnosis and treatment of IBS were mostly conducted by synthetic medicines including antispasmodic drugs that have many side effects and are so expensive. The aim of the present study was to investigate the antispasmodic efficacy of polyherbal formulation prepared using extract of Abies webbiana, Plumbago zeylanica and Rubia cordifolia with excipients. Antispasmodic effectiveness of polyherbal formulation was assessed by inhibition of the spasams induced by spasmogens. The spasmolytic activity of polyherbal formulation was studied in isolated rats’ileum model using histamine as agonist, which later compared to chlorpheniramine maleate as reference drug. The evaluation parameter was studied started with the physical test such as nature, colour, odour and texture. The all the three formulations were liquid in nature and dark brown in colour with pleasant odour. The physicochemical parameters of formulation studied using sedimentation volume, particle size, viscosity, pH, redispersibility, zeta potential and density. The sedimentation volume ranged from 0.21 to 0.29. The pH of formulations F1 to F3 ranged from 5.10 to 5.27 and the viscosity ranged from 43 to 47 cps. The maximum viscosity was observed in F1 formulation (i.e. 47.3 cps). Zeta potential values were in the order of F2>F1>F3. The density of suspensions was in range of 1.06 to 1.14.On basis of these physiochemical parameters F2 formulation have best properties.  The histamine (10µg/ml) produced dose dependent contraction of rat ileum. The RCM, AWM, PZM and PHF F2 significantly inhibited (p<0.05) the contractile effect of histamine on isolated rat ileum preparation. The percentage relaxation of PZM on histamine induced contraction was found to be 88.68%. Similarly, the percentage relaxation of F2 Formulation on histamine induced contraction was found to be 89.90% in comparison with chlorpheniramine CPM (10µg/ml) 96.02%. Therefore, the PHF-F2 showed almost similar antihistaminic effect that of chlorpheniramine maleate.