Phyto-Mediated Synthesis Of Copper Nanoparticle Using Seabuckthorn (Hipphophae Rhamnoides L.) Stem Extract And Assessment Of Its Antioxidant Activities

Abstract

The exceptional properties of green synthesized copper nanoparticle like its sustainable nature, non-toxic, cost effective, pollution free as well as environmentally friendly nature of copper nanoparticle and its different application in the field of medical science made copper nanoparticles (Cu-NPs) more demanding among the researchers. In the present investigation copper nanoparticles have been synthesized from Seabuckthorn (Hipphophae rhamnoides L.) stem extract which is also known as greener method and their corresponding characteristization were studied using UV–visible spectroscopy, XRD, FT-IR, SEM analysis. CuSO₄ was reduced to copper nanoparticles and is confirmed by UV–visible spectrophotometer analysis. The maximum absorption occurring at 384 nm at the visible spectrum of UV rays confirms the surface plasmon resonance of the nanoparticles. The result of the FTIR spectroscopy analysis of the nanoparticles complements the involvement of functional groups or biomolecules of the stem extract in the synthesis. The synthesized particles were extremely durable, spherical with the average particle size in the range of 13 ± 50 nm. The anti-oxidant activity is also increased when CuNPs of Hippophae rhamnoides L., stem such as FRAP at 100 µl gave 269µM of its concentration and POD and SOD with 0.69µM/L/gm dw/sec. and 0.12 U/ml of concentration. These results show that Seabuckthorn (Hippophae rhamnoides L). stem extracts synthesized copper nanoparticle having significance anti-oxidant properties and could be used as possible drugs to prevent ailments.