Evaluation Of Antimicrobial Activity And Antioxidant Potential Of Green Synthesized Silver Nanoparticles With Apple Cider Vinegar

Abstract

Plant-mediated biological formation of NPs has turned out to be more significant lately due to its ease of use and environmental friendliness. This study reported the utilization of apple cider vinegar for the synthesis of silver NP (AgNPs) to determine their antimicrobial and anti-oxidant activities. The bio-synthesized silver nano-particles were validated through various characterization methods like XRD, FTIR, UV-Vis spectroscopy, and SEM. The antioxidant property of the bio-synthesized AgNPs was studied through various assays like peroxides (POD), Superoxide dismutase (SOD) and Ferric Reducing Antioxidant Power Assay (FRAP) assay. The antimicrobial activity was carried out against the Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis and fungus isolates of Candida albicans, Penicillium chrysogenum, Asperigillus niger and Trichoderma reesei.The antioxidant property of the sample increased with the volume of the sample during the FRAP assay where the highest concentration was observed to be 702.63 µM at 100 µl. The POD and SOD activity of the sample were observed to be 0.675 µM/L/gmdwt/sec. and 0.16 U/ml. During the antimicrobial activity, the synthesized AgNPs from apple cider vinegar showed great inhibition against of pathogenic bacteria and fungi at a concentration of 80 µl. The apple cider AgNPs inhibited the bacteria at different concentrations with higher antibacterial activity as follows: P. aeruginosa >E. coli > S. aureus >B. cereus whereas against all the fungal strains ZOI was 8 mm except T. reesei (10 mm) when treated with the AgNPs synthesized by apple cider vinegar. The data strongly suggest that the apple cider vinegar AgNPs have a significant antioxidant and antimicrobial potential.