CHARACTERIZATION, QUANTIFICATION, AND ESTABLISHMENT OF COEFFICIENT EXTINCTION OF RABIES ANTIGEN SAMPLES USING DIFFERENT ANALYTICAL METHODS

Abstract

The current paper is related to the survey on the Characterization, Quantification, and Establishment of Coefficient Extinction of Rabies Antigen Samples Using Different Analytical Methods. Diagnostic tests will determine whether or not rabies has been contained. Early disease detection is critical for containment and preventing the disease from spreading to other suitable vectors. Because it can be transmitted through the skin and mucous membranes via saliva, strong barrier measures will be required. The fact that a disease condition is irreversible once symptoms appear, the existence of various virus variants, and the presence of wild animal reservoirs all increase the risk of transmission to humans when they come into contact with domestic animals. All of these problems highlight the necessity of establishing a system to track the spread of rabies among wild animals. Standard methods (MIT, RTCIT) and one recommended procedure (RREID) for rabies diagnosis are compared to determine the threshold value, i.e. the highest dilution of samples for these methods that can be recognized as positive for rabies infection. Additionally, the WHO recommends rapid rabies enzyme immune diagnosis (RREID), which allows for the examination of larger numbers of samples and provides results in 3–4 hours. As opposed to MIT, which has a higher threshold for acceptance, RREID allows researchers to examine a large number of samples quickly and visually without the use of a spectrophotometer, which makes it ideal for laboratories with less sophisticated equipment. RT-high PCR's sensitivity was confirmed in our experiments by literary data as well as our own findings. A more sensitive diagnostic procedure, Nested RT-PCR, was discovered than RREID, MIT and RTCIT, with a threshold value of dilution 10–4 compared to RREID's only 10–2.