Molecular Study Of Antibiotics Resistance Klebsiella Pneumoniae Genes Isolates From Urinary Tract Infection

Abstract

This study was completed in laboratories of Biology Department in Faculty of Science. It explains of antibiotics resistance Klebsiella pneumoniae that isolated from urinary tract infection patients in the province of Thi Qar. A total number of (200) samples were collected from patients with urinary tract infection from AL-Hussein Teaching Hospital and AL- Shatrah General Hospital in Thi-Qar Governorate, Klebsiella pneumoniae isolated from urinary tract infection revealed that 65 K. pneumoniae isolates against 15 commonly used antibacterial agents by using the disk diffusion method K. pneumoniae isolates has a great resistance to most commonly antibiotics  used in treatment of urinary tract infection, the highest rate of resistance is seen with Ampicillin and Vancomycin 65/65 (100%) followed by Trimethoprim 53/65 (81.53%), Ceftriaxone 47/65 (72.30%) and Tetracycline 39/65 (60%). The results showed that the (GyrA) gene was detected in K. pneumonia isolates 40/40 (100%), from the 40 (100%) isolates, the result exposed 34/40 (85%) positive isolates of K. pneumonia for the (blaSHV) gene, the results also displayed the (blaVIM) gene was detected in K. pneumonia  isolates, 22/40 (55%) were have (blaVIM) gene, (sul1) gene was detected in K. pneumonia isolates 18/40 (45%), the results indicated 16/40 (40%) positive isolates of K. pneumonia for the (Ant(2")-1a), (rmtB) gene was detected in K. pneumonia isolates, 15/40 (37.5%) were have (rmtB) gene, the (qnrA) gene was detected in K. pneumonia isolates 12/40 (30%), the results displayed 8/40 (20%) were have (blaIMP) gene, regarding (blaKPC) gene the result presented that the (blaKPC) gene was detected in K. pneumonia isolates, 6 (15%) were have (blaKPC) gene, the (ermB) gene was detected in K. pneumonia isolates 3/40 (7.5%).