Phytochemical Screening, Antioxidant Potential, Isolation And Characterization Of Bioactive Compound From Enhalus Acoroides

Abstract

Background: Naturally derived bioactive compounds from marine sources such as seagrasses possess bio-pharmaceutical agents. The current study focusses on qualitative, quantitative analysis of phytochemical, in-vitro antioxidant activity of different extracts, isolation and identification of compound from Enhalus acoroides. The qualitative analysis reveals abundant presence of alkaloids, flavonoids, coumarin, glycosides, terpenoids, tannin, steroid, saponin and phenol in Hydroalcoholic extract. Quantitative analysis of total alkaloid, flavonoid, phenol, tannin and terpenoids were determined on Hydroalcoholic extract of Enhalus acoroides by spectrophotometric methods. The yield was flavonoid (121.26 ± 8.48 mg QE), phenol (206.08 ±14.42 mg GAE), tannin (88.22 ± 6.17 mg TAE) and Saponin (65.20 ± 4.56 mg QUE). Hydroalcoholic extract of Enhalus acoroides was evaluated for in-vitro antioxidant assays.  IC₅₀ value for DPPH radical scavenging activity, Total antioxidant activity, Hydroxyl radical scavenging activity, Superoxide anion scavenging activity and Nitric oxide scavenging activity were (40.81µg/ml), (41.82µg/ml), (40.54µg/ml), (41.34µg/ml), (39.42µg/ml) respectively. Hydroalcoholic extract showed good results in scavenging free radicals. Isolation and characterisation of Bioactive compounds were performed by Column Chromatography, TLC, UV, FT-IR, Mass spectrum and NMR study. The fractions obtained from column chromatography are Hexane, Chloroform and Methanol: Chloroform. On basis of R_f value in TLC separation the isolated compound was a flavonoid derivative. FT-IR analysis shows the presence of functional groups of isolated compound. ¹H-NMR Spectrum of Methyl gallate confirms the signals of protons. The ¹³C -NMR Spectrum of Methyl gallate was inveterate by the 8 carbon signals. On basis of NMR study, the isolated compound was confirmed as Methyl gallate (C₈H₈O₅).