A Validated Stability Indicating Simultaneous Quantitation Of Pyrimethamine And Sulphadoxine In Combined Tablet Dosage Form Using Diffuse Reflectance Infrared Fourier Transform Spectroscopy

Authors

  • Nalanda Rangari , Saroja Naubade , Kalyani Jadhav , Sanjay Chaudhari , Madhavi Kapale , Tukaram M. Kalyankar and Omprakash A. Khogre

DOI:

https://doi.org/10.47750/pnr.2022.13.S09.306

Abstract

A simple, precise, rapid, economic and eco-friendly stability indicating simultaneous quantitation of pyrimethamine and sulphadoxine in combined tablet dosage form using diffuse reflectance infrared fourier transform spectroscopy was developed and validated. The wave numbers selected were in range of 3242-3145 cm-1 (NH) for pyrimethamine and 1354-1311 cm-1 (S=O) for sulphadoxine. Beer-Lambert’s law followed in concentration range of 0.2-1.2 % w/w and 4-24 % w/w with good correlation coefficient of 0.998 and 0.997 for pyrimethamine and sulphadoxine respectively. The method has good reproducibility with % RSD less than two. The proposed method was validated for accuracy, precision, linearity, limits of detection (LOD), limits of quantitation (LOQ) and reproducibility according to ICH guidelines. The percent recovery of pyrimethamine and sulphadoxine in marketed dosage form was in range of 99.13 ±0.3971 and 99.69 ±0.3100 respectively. Only solid state stability was performed like photolysis, thermal and sunlight degradation. Thus the proposed method can be applied for simultaneous estimation of pyrimethamine and sulphadoxine in pharmaceutical dosage form and in bulk.

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Published

2022-11-24 — Updated on 2022-11-24

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Nalanda Rangari , Saroja Naubade , Kalyani Jadhav , Sanjay Chaudhari , Madhavi Kapale , Tukaram M. Kalyankar and Omprakash A. Khogre. (2022). A Validated Stability Indicating Simultaneous Quantitation Of Pyrimethamine And Sulphadoxine In Combined Tablet Dosage Form Using Diffuse Reflectance Infrared Fourier Transform Spectroscopy. Journal of Pharmaceutical Negative Results, 2574–2585. https://doi.org/10.47750/pnr.2022.13.S09.306

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