Molecular Characterization and Phylogenetic Analysis of Colletotrichum cobbittiense in Cyanthillium cinereum (Vernonia cinerea)
Keywords:Pathogen, ITS-rDNA, Cyanthillium cinereum, Colletotrichum cobbittiense.
Cyanthillium cinereum, also known as Vernonia cinerea, is an annual plant native to tropical Asia and a member of the Asteraceae family. In India, the plant is almost universally considered a noxious weed. Recent surveys in Chelluru Village in the East Godavari district of India have revealed the appearance of a leaf spot on the host plant. The infested leaves are collected in this area and are brought to the laboratory for examination. The objective of this work was to determine not only the molecular characterization and phylogenetic analysis of the pathogen, but also the pathogen responsible for the disease affecting Cyanthillium cinereum (Vernonia cinerea). The molecular approach is used to determine the identity of the fungus. Using sequencing data from the ITS -rDNA region, the identification of the species was confirmed to be correct. The phylogenetic tree was constructed using the Maximum Parsimony (MP), Maximum Likelihood (ML) and Neighbor Joining (NJ) approaches. Colletotrichum cobbittiense was determined to be the pathogen in question. In Cyanthillium cinereum, the pathogen Colletotrichum cobbbitiense was detected for the first time. Under controlled laboratory conditions, the pathogenicity of Colletotrichum cobbittiense was demonstrated by inoculation of leaves of Cyanthillium cinereum. The effects of the disease and the course of its life are still being researched.