Formulation And Evaluation Of Hydrogel Containing Martynia Annua And Ocimum Sanctum Leaves Extract For Anti-Inflammatory Activity
Objective: The purpose of this research was to develop a hydrogel formulation of the methanolic fraction of Martynia annua and Ocimum sanctum and evaluate its potential anti-inflammatory effects in a number of animal models.
Materials and Methods: The leaves of Martynia annua and Ocimum sanctum were used in the preparation of six unique hydrogel formulations, five of which contained extract, and one of which did not contain any extract at all. This was done in accordance with the standard method, and different proportions of Carbopol 940 and sodium carboxymethyl cellulose (CMC) were used. The prepared hydrogel was evaluated for optimization purposes and assessed for anti-inflammatory efficacy in mice with ear edema caused by Xylene and Croton oil, respectively. The effect was seen by the assessment of the percent inhibition of ear edema as well as biochemical indicators such as the level of nitric oxide and the level of myeloperoxidase (MPO).
Results: Results from a phytochemical analysis of M. annua and o. sanctum petroleum ether extract revealed the presence of sterols, terpenoids, and fatty oils, while results from an analysis of ethanol extract of both plant leaves revealed the presence of glycosides, phenolic compounds, flavonoids, and amino acids. Hydrogels produced from the methanol fraction of O. sanctum and M. annua have been shown to have anti-inflammatory properties, according to the current investigation. The findings that croton oil may stimulate neutrophil recruitment to mice ear tissue. Fortunately, Voltaren Emulgel and extract hydrogels were shown to considerably (P<0.01) lower MPO levels and NO levels in the ears of mice, suggesting that they might help solve the issue.
Conclusion: Ear edema caused by xylene was inhibited by a methanol fraction of leaves from M. annua and Ocimum sanctum. This may be because the extract blocks the production, release, or activity of xylene, a compound thought to have a role in the inflammation. Inhibiting the production, release, or activity of histamine is how methanol extracts show their anti-inflammatory effects. Flavonoids in the methanol extract fraction of M. annua and Ocimum sanctum leaves may account for their noteworthy activity.
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