Comparison Of Current Diagnostic Methods And Molecular Identification Of Pneumocystis Jirovecii Causing PJP In Cases Immune Suppressed Patients At The Nephrology Ward, Imam Khomeini Hospital, Urmia

Authors

  • Negar Javanmard Barbin , Khosrow Hazrati Tappeh , Kambiz Diba , Khadijeh Makhdoumi , Shahram Khademvatan

DOI:

https://doi.org/10.47750/pnr.2022.13.S09.235

Abstract

Background and purpose: Pneumocystis pneumonia caused by Pneumocystis jirovecii (PJP), is an opportunistic life-threatening infection for immunocompromised individuals; including both human immunodeficiency virus (HIV)-infected and HIV-uninfected patients treated with immunosuppressive medications. Immunocompetent people can also be a spreading agent for PJP, therefore assessment of colonization is of great significance. Considering the scarcity of published reports on Pneumocystis jirovecii isolates from patients in northwest of Iran; we aimed to diagnose and identify Pneumocystis jirovecii in respiratory samples obtained from hospitalized immunosuppressed patients in Imam Khomeini hospital, Urmia using Giemsa and Grocott methenamine silver (GMS) staining along with molecular method. The current study also would be used to compare the diagnostic methods for detection of Pneumocystis jirovecii.

Materials and Methods: During a period of 15 months (February 2019 until June 2020), 200 respiratory samples (175 Bronchoalveolar lavage and 25 sputum) were collected from hospitalized patients receiving prolonged immunosuppressive therapies (kidney transplant recipients, patients with autoimmune disorders and malignant disorders under chemotherapy). In the following, detection of Pneumocystis jirovecii was performed for each sample using Giemsa and GMS staining method and also nested PCR assay targeting the mitochondrial large subunit ribosomal RNA (mt LSU rRNA) gene.

Results: The results of direct microscopic examination after Giemsa staining revealed the presence of Pneumocystis jirovecii in 3 out of 200 (1.5 %), and of all clinical samples only 6 cases (3 %) were positive by GMS staining. In addition, DNA of Pneumocystis jirovecii was detected in 12 out of 200 (6 %) respiratory samples.

Conclusion: According to the present findings, the frequency of PJP was similar to the rates reported in other similar studies in Iran. Furthermore, nested PCR might be a reliable technique for diagnosis of Pneumocystis jirovecii as compared with the direct staining methods.

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Published

2022-11-21 — Updated on 2022-11-21

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How to Cite

Negar Javanmard Barbin , Khosrow Hazrati Tappeh , Kambiz Diba , Khadijeh Makhdoumi , Shahram Khademvatan. (2022). Comparison Of Current Diagnostic Methods And Molecular Identification Of Pneumocystis Jirovecii Causing PJP In Cases Immune Suppressed Patients At The Nephrology Ward, Imam Khomeini Hospital, Urmia. Journal of Pharmaceutical Negative Results, 1953–1966. https://doi.org/10.47750/pnr.2022.13.S09.235

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