Chemopreventive Anticancer Activity Of Beta Sitosterol In Human Prostate Cancer Cells (Pc-3) And Emt-Mediated Signaling Regulation: An In Vitro Study

Authors

  • Chevuru Sai Shreya Reddy , Dr. R Priyadharshini , Dr. Selvaraj Jayaraman , Dr. Palati Sinduja

DOI:

https://doi.org/10.47750/pnr.2022.13.S09.185

Abstract

Background: Cancer is one of the leading causes of death around the world. It has potential to spread and destroy the surrounding tissues. The most common cancer seen in men is prostate cancer. β-sitosterol is a white colored, waxy powdered sterol found naturally in plants. It has shown to inhibit the activity of prostate cancer. Aim of this study is to analyze the anticancer activity of β-sitosterol in human prostate cancer cells.

 Materials and Methods: Trypsin-EDTA, fetal bovine serum (FBS), antibiotics-antimycotics, Dulbecco’s modified Eagle’s medium (DMEM) and phosphate buffered saline (PBS) are used as materials. The cell line used is PC-3. MTT test was used to check cell viability by calorimetric technique. The HT 29 cell was exposed to different mediums. Then the cell viability is calculated. Real time PCR was used to analyze gene expression in which cDNA synthesis was performed in varied sample volumes. Melting curves were acquired for all samples. Data was analyzed through comparative CT method and by graph pad prism.

 Results: In PC3 cells, the effect of β-sitosterol extract on the percentage of viable cells, Bcl-xL mRNA, BCL-2 mRNA and Mcl-1 mRNA expression were analyzed with mean ± SD at various concentrations which showed significance of p<0.05.

 Conclusion: From this study, we can conclude that β-sitosterol acts as an anticancer potential when induced in prostate cells, decreasing the rate of cells through EMT signaling molecules.

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Published

2022-11-18 — Updated on 2022-11-18

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How to Cite

Chevuru Sai Shreya Reddy , Dr. R Priyadharshini , Dr. Selvaraj Jayaraman , Dr. Palati Sinduja. (2022). Chemopreventive Anticancer Activity Of Beta Sitosterol In Human Prostate Cancer Cells (Pc-3) And Emt-Mediated Signaling Regulation: An In Vitro Study. Journal of Pharmaceutical Negative Results, 1514–1521. https://doi.org/10.47750/pnr.2022.13.S09.185

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