Morphological And Physiological Responses To Npcrna Pmir-137 Knockout In Proteus Mirabilis

Authors

  • Abirami Sanniraj
  • Suresh V. Chinni
  • Sumitha Samuggam
  • Eugene Boon Beng Ong
  • Antony V Samrot
  • Lee Su Yin

DOI:

https://doi.org/10.47750/pnr.2022.13.S09.82

Abstract

Proteus mirabilis is a Gram-negative, facultatively anaerobic rod-shaped bacterium, known for its ability to form multicellular surface mobility termed swarming. Proteus mirabilis causes symptomatic urinary tract infections, such as cystitis and pyelonephritis, and catheter-associated urinary tract infections. There are several virulent factors associated with P. mirabilis such as flagella, fimbriae, hemolysin, urease, and outer membrane proteins. It uses flagella-mediated motility in order to reach the ureters and renal parenchyma in the host urinary system, eventually causing urinary tract infection. Flagella are hollow tubes composed of a long helical filament, a small, curved structure (the hook) and the basal body embedded on the cell surface which plays a significant role in bacterial movement through aqueous environment. Our previous study revealed 240 novel Hfq-associated nonprotein coding RNAs in P. mirabilis. Interestingly, npcRNA PmiR-137, the Hfq-associated novel non-protein coding RNA is predicted to regulate the virulence associated with the mRNA of flagella proteins. Thus, the PmiR-137 was knocked out to study the morphological and physiological differences between the wild-type and mutant strain. The mutant strain exhibited higher motility and biofilm production compared to the wild-type strain. The mutant strain was unable to survive heat shock and oxidative stress conditions, resulting in a lower percentage of relative survival rate. However, no morphological differences could be observed between the wild-type and mutant strain.

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Published

2022-11-13 — Updated on 2022-11-13

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How to Cite

Abirami Sanniraj, Suresh V. Chinni, Sumitha Samuggam, Eugene Boon Beng Ong, Antony V Samrot, & Lee Su Yin. (2022). Morphological And Physiological Responses To Npcrna Pmir-137 Knockout In Proteus Mirabilis . Journal of Pharmaceutical Negative Results, 723–729. https://doi.org/10.47750/pnr.2022.13.S09.82

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