Comparative Evaluation Of Olive Oil And Coconut Oil With Xylene As A Clearing Agent In Histopathology

Abstract

Aim: The aim of the present study was to evaluate the efficacy of olive oil and coconut oil as clearing agent with regards to Xylene and to compare the efficacy of olive oil and coconut oil as clearing agent in two different histopathological staining methods.

Methods: The study was carried out in Department of Oral Pathology & Microbiology, Darshan Dental College & Hospital, Loyara (Udaipur), Rajasthan. A total of 192 tissue specimens were collected, fixed in 10% formalin, and sectioned into 8 equal parts in each group broadly divided in 6 grouped as group I, II, III, IV, V, VI Groups were divided on the clearing agents and stains used respectively. Tissue specimen were taken for routine processing followed by H&E and PAS staining procedure with xylene in group I & II as clearing agent, whereas group III & IV have Coconut oil as clearing agent and group V & VI have Olive oil as clearing agent.

Results: Coconut and Olive Oil treated specimen showed better characteristic features than Xylene treated specimen with respect to rigidity, tissue shrinkage and translucency respectively with p (<0.001). Both Coconut and Olive oil treated specimen showed similar features in almost all parameters on both macro and microscopic levels with insignificant p value of (>0.05). Among H&E and PAS-stained slides, coconut and Olive oil sections showed better nuclear staining, cytoplasmic staining and quality of staining compared to Xylene processed sections.

Conclusion: Coconut and Olive oil treated specimen showed better characteristic features than xylene treated specimen with respect to rigidity, tissue shrinkage and translucency. Coconut and Olive oil treated sections showed better nuclear staining, cytoplasmic staining, and quality of staining than Xylene treated sections.